Changes in Soil Properties, Bacterial Communities and Wheat Roots Responding to Subsoiling in South Loess Plateau of China

This study was carried out to investigate effects of subsoiling on the diversity and composition bacterial community in a wheat–maize rotation field Guanzhong area Shaanxi Province, China. After wheat harvest, surface soil samples were collected under two tillage methods (single rotary (RT) + (ST)) perform high-throughput sequencing bioinformatics analysis. Soil properties root length density (RLD) winter at booting flowering stages also studied. Results showed that ST treatment significantly raised water storage, organic carbon total nitrogen contents deep (>40 cm), notably increased pH, ammonium content RLD layer from 0–70 cm stage 0~100 stage, but residual nitrate decreased by 17.74%. Compared with RT, richness 10~20 trend. The relative abundances GAL15, Actinobacteria, Nitrospirae, Rhizobiales, Burkholderiales, Pseudomonas Serratia 10–20 remarkably ST. Principal Component Analysis (PCA) Redundancy (RDA) results have strongest effect structure. In addition, there positive correlations between content. conclusion, although not conducive improving diversity, it beneficial bacteria (biological nitrogen-fixing bacteria, ammonifying nitrobacteria) abundances, reduced loss caused denitrifying promoted earlier development improved plant utilization ratio nutrients.